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Lab experiments
Slide preparation to see the internalization of nanoparticles in HeLa cells
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The purpose of this research is the preparation of some slides to see the internalization of nanoparticles (doxorubicin and cisplatin) in tumour cells under the microscope.
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https://www.youtube.com/watch?v=cWuahy1EPHM
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Observation of the internalization of nanoparticles under the confocal microscope
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This research is prepare a capsule with HeLa cells and nanoparticles of cisplatin and two capsules with HeLa cells and nanoparticles of doxorubicin and cisplatin in different concentrations to see under the confocal microscope the internalization of theses nanoparticles in HeLa cells.
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https://www.youtube.com/watch?v=qEGTqRQ7-w4&t
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Cytotoxicity proofs of nanoparticles of doxorubicin and cisplatin
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The objective of this research is to sow HeLa cells with nanoparticles in a capsule with 96 tiny wells in order to find out the IC50, the point where, in a determined concentration, half of the cells are dead, and the other half alive.
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https://www.youtube.com/watch?v=UI4MzgRgrDs&t
Chemotherapeutic Drugs
Cisplatin
Chemotherapy medication used to treat a number of cancers such as testicular cancer, breast cancer or brain tumors. Cisplatin interferes with DNA replication, which kills the fastest proliferating cells, which in theory are carcinogenic. One of the two chloride ligands is displaced by water (aquation),so the platinum atom can be inserted into a DNA. As a result, crosslinking occurs between two nucleobases of DNA during the process of replication.
Doxorubicin
Chemotherapy medicine used in a lot of types of cancer as leukemia and lymphoma. Doxorubicin is in the anthracycline and antitumor antibiotic family medications. Doxorubicin interacts with DNA by intercalation and inhibition of macromolecular biosynthesis. This inhibits the progression of the enzyme topoisomerase II, which relaxes supercoils in DNA for transcription. Doxorubicin stabilizes the topoisomerase II complex after it has broken the DNA chain for replication, preventing the DNA double felix from being resealed and thereby stopping the process of replication.
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